MorecosteffectivethanEDTA

• OptimizedforDNAmethylation,PCRandNGSassays

• PreserveandMaintainSampleIntegrityfor3months

• CompatIBLewithmultipleextraction/purificationtechniques

• Compatiblewithalldownstreamapplications

Overview

DNAgard™BloodTubeisdesignedforthecollection,preservationandshippingofwholebloodsamplesforgenomicDNAisolation.Thepreservativereagentrapidlypermeatescellularstructuresandmembranes(celllysis)tostABIlizeandprotectgenomicDNAinwholebloodsamples.TheuseofDNAgardBloodTubeprovidesastreamlinedworkflowfrombloodcollectionintheclinicorfieldtosampleprocessinginthelaboratory.

Collect,shipandpreserveDNAinbloodunderoptimalconditions
RecoverhighqualityDNA(highMWfragments)
RecoverquantitativeDNA(yield)

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ProductData

ProtectDNAatambienttemperatures

Figure-1:DNAgardBloodTubeformulationpreservesDNAinwholebloodfor3months.GelimagesofisolatedgenomicDNArecoveredfrombloodsamplespreservedfor14monthsinDNAgardBlood(DGB)atroomtemperature,inacompetitorpreservative(COM)atroomtemperature,unprotected(NP)atroomtemperature,versuscontrol(-20°C).

Broadrangeofapplications

DNAprotectedusingtheDNAgardBloodTubeformulationissuitableforuseinmanydownstreamapplications,e.g.long-rangePCR,realtimePCR,etc.

Figure2A.SuccessfulLong-rangePCRafter2monthsstorage.GenomicDNAisolatedfromhumanwholebloodstoredfor60daysintheDNAgardBloodTubeformulation,non-protected(NP)orfrozenat-20°C.Long-rangePCRamplificationofa22KbpampliconwasperformedontheDNA(M=1kbladder).Figure2B.Real-timePCRafter1monthstorage.GenomicDNAisolatedfromhumanwholebloodstoredfor30daysintheDNAgardBloodTubeformulationorfrozenat-20°Cwasquantifiedusingreal-timePCRamplificationoftheb-actingene.

RepresentativeDNAyield

DNAprotectedusingtheDNAgardBloodTubeformationissuitableforuseinmanydownstreamapplications,e.g.long-rangePCR,realtimePCR,etc.

Figure3.qPCRquantificationafter1monthstorageofblood,from3differentdonors,inDNAgardBloodTubeformulation(RT),Competitor(RT)orfrozen(-20°C).

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Specifications

Evacuated,sterile,bloodcollectiontubes(10ml)
Pre-fillvolume(preservative)=1.0ml
Blooddrawvolume=6.5ml
Storagetemperature=-20°Cto45°C
BloodDNApreservation=3monthsatroomtemperature
ForResearchUseOnly.Notforuseindiagnosticprocedures.

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DNAgardBloodTubes

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