StABIlizeandPreserveDNAinWholeBloodatAmbientTemperature
- PreserveDNAinbloodandbuffycoatsamplesforatleast12yearsatambienttemperatures.
- Costeffectiveandconvenientbloodsamplearchiving
- CompatIBLewithalldownstreamapplications
Product
CostEffectiveSolutionstoStoreBloodSamples
DNAstāble™ BloodisaninnovativeproductfordrystorageandpreservationofDNAinbloodsamplesatambienttemperatures.TheDNAstableBloodmatrixformsaThermostablebarrierassamplesdry,protectingthebloodDNAfromdegradation.DNAstableBloodisavailablepre-driedintubes.OncetheDNAstableBloodreagentismixedwithblood,samplescanbedriedinalaminarflowhoodatroomtemperature,inaventilated40°Cincubator,orinavacuumcentrifuge.Samplescanthenbestoredatambienttemperatureforatleast12years(basedonacceleratedagingstudies).
| Properties | Biomatrica | FTACard | ColdChain |
|---|---|---|---|
| Yield | |||
| Recovery | |||
| DNAProfiling | |||
| LowConcentrationDNA | |||
| FormatFlexibility | |||
| ShippingRobustness | |||
| CostEffectiveness |
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Data
NodegradationofbloodDNAafter12yearsofstorage*
Figure1.Humanwholebloodsamples(200µl)werestoredfor31monthsundervariousconditions:storageat–20°C(Control);storageinDNAstableBloodat25°Cor45°C(equivalenttoapprox.12yearsofstorageatroomtemperature)1;orstorageat45°Cwithoutprotection(NP).Afterbloodrehydration,genomicDNAwaspurifiedusingtheQIAampDNABloodMiniKit.DNAeluates(10µl)wereanalyzedona0.8%agarosegel.M:Markers.
HigherDNArecoveryfromwholebloodstoredinDNAstableBloodvsFTApaper
Figure2.DuplicateHumanwholebloodsamples(200µl)werestoredfor12monthsundervariousconditions:storageat–20°C(Control);storageinDNAstableBloodat45°C(equivalenttoapprox.5yearsofstorageatroomtemperature);*orstorageat25°ConFTApaper(FTA).Afterbloodrehydration,genomicDNAwaspurifiedusingtheQIAampDNABloodMiniKit.DNAeluates(10µl)wereanalyzedona0.8%agarosegel.M:markers.extraction.(M=1kbladder).
NodegradationofbuffycoatDNAafter11yearsofstorage*
Figure3.Humanbuffycoatsamples(200µl)werestoredfor27monthsundervariousconditions:storageat–20°C(Control);storageinDNAstableBloodat45°C(equivalenttoapprox.11yearsofstorageatroomtemperature)1.Afterbuffycoatrehydration,genomicDNAwaspurifiedusingtheQIAampDNABloodMiniKit.DNAeluates(10µl)wereanalyzedona0.8%agarosegel.M:markers.
BroadRangeofApplications
Successfullong-rangePCRafter3yearsofstorage*
Figure4.Wholebloodsamples(200µl)fromthesamedonorwerestoredfor9monthsat–20°CorinDNAstableBloodTubesat25°Cor45°C(equivalenttoapprox.3yearsofstorageatroomtemperature).*Afterbloodrehydration,genomicDNAwaspurifiedusingtheQIAamp®DNABloodMiniKit.DNAeluates(2µl)werethenusedinPCRofa5kbDNAfragmentofthehumangenomeusingtheQiagen®LongRangePCRKit.ThePCRproductswerevisualizedona0.8%agarosegel.M:1kbPlusLadder.
Reliablepolymorphismdetectionusingmeltingcurveanalysisafter9monthsofstorage
Figure5.Humanwholebloodsamples(200µl)werestoredfor9monthsat–20°CorinDNAstableBloodTubesatroomtemperature.Afterbloodrehydration,genomicDNAwaspurifiedusingtheQIAampDNABloodMiniKit.DNAeluates(2µl)werethenanalyzedusingtheartus®MTHFRLCPCRKit.‡Bothsamplesshowednosignificantdifferenceinmeltingcurveanalysisandindicatedaheterozygousvariant.
*Acceleratedagingstudies:Atemperatureincreaseof10°Ccorrespondstoatwofoldincreaseinshelflife.Forexample,DNAstoredat40°Cfor2yearscorrespondstoanestimatedstoragetimeof8yearsat20°C.
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Specifications
- Productformats: Tubes
- Applicationvolume:1–200µLofblood
- DNARecovery: ~99.9%
- BloodDNAlong-termstorage: Atleast12yearsatambienttemperatures
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