StABIlizeandstorepurifiedDNAatRoomTemperature
- NextgenerationliquidpreservativeforpurifiedDNA–atalltemperatures
- LiquidformatoptioncompatIBLewithautomationandsamplehandlingworkflows
- RecoverhighamountsofDNAandanalyzeinstantlywithoutfurtherpurification
- Compatiblewithmostdownstreamapplications,e.g.,PCR,RT-PCR,SNPanalysis,DNAsequencing,etc.
- Cost-effective,eco-friendlysolutiontofreezercrawlandlabspacemanagement
Product
DNAstāble™Plusenablesyoutopreserve,storeandshipyourpurifiedDNAsamplesatroomtemperatureinliquidandliquid-to-dryformats.ItoffersflexibilitywithThermostabilityatextremetemperatures,andtheabilitytohandlehighconcentrationsofDNA.Recoveryfromthedrymatrixisachievedwithinminutesbysimplyaddingwater.YourDNAsampleisreadyfordownstreamanalysiswithouttheneedforanyadditionalpurificationsteps.
| Properties | Biomatrica | Unprotected | ColdChain |
|---|---|---|---|
| Yield | |||
| Stability | |||
| QualityofAmplification | |||
| LowConcentrationDNA | |||
| ShortTermStorage<1> | |||
| LongTermStorage>1year | |||
| ShippingRobustness | |||
| CostEffectiveness |
DNAstabilizationcanbeachievedintwoformats:
- LiquidStabilization(short-term)
- Liquid-to-Dry(LD)Stabilization(long-term)
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Data
StabilityinLiquidFormat:
Figure1:ThegelimageaboveshowsprotectionofDNAsamplesstoredinDNAstablePlus(conc:50ng/ul)for6monthsat45°Cinliquidformatcomparedtounprotectedsamples(TE;novisiblebands).Thepositivecontrolsaresamplesstoredat4°C.
StabilityinLiquid-to-Dry(LD)Format:
Figure2:Stabilityfor3yearsat60°CSamples1-3and10-12aboverepresentDNAstoredinTEbufferfor3yearsat4°Cinliquidformat(positivecontrol).Theremainingsampleswerestoreddryat60°Cfor3years.Samples4-9represent50ng/µLDNAstoredineitherDNAstablePlus(4-6)orTE(7-9).Samples13-18represent5ng/µLDNAstoredineitherDNAstablePlus(13-15)orTE(16-18).100ngofthetriplicatesamplesperconditionwererunona1%agarosegelstainedwithethidiumbromide.
Figure3:Stabilityfor6monthsat85°CThegelimageaboveshowsprotectionofDNAsamplesstoredinDNAstablePlus(conc:50ng/ul)for6monthsat85°Cinliquid-to-dryformatcomparedtounprotectedsamples(NP;novisiblebands).Thepositivecontrolsaresamplesstoredat4°C.
Figure4:Long-rangePCRassaysusingDNAsamplesstoredfor3yearsinDNAstablePlussamples1-3aboverepresentDNAstoredfor3yearsinTEbufferat4°Cinliquidformatwhiletherestofthesampleswerestoreddryat60°Cfor3yearsat50ng/µL.Samples4-6representDNAstoredinDNAstablePlus,whilesamples7-9representDNAstoredinTEbuffer.100ngofrehydratedDNAfromtriplicatesampleswereamplifiedusingKODXtreme™HotStartPCRkit(Novagen)with7.5kbhumanβ-globinprimers.
TechnicalData:
- DownstreamAnalysis(qPCR)ofgenomicDNAstoreddryfor6monthsatvarious“STRESS”conditions.
- DNAstablePlusprotectsgenomicDNAunderextremestressconditionsat+85°CinLDformat.
- DNAstablePlusdoesnotcauseanyinterferencewithdownstreamanalysis.
- DNArecoveredfromsamplesprotectedwithDNAstablePlus(stressedat60°Cor85°C)aresimilartoDNArecoveredfromstoredat4°C.
Figure5:HumangenomicDNAsampleswerestoredfor6monthsinanumberofconditions,recoveredandcompared.Fromlefttoright:Control4°C;StoredinDNAstablePlusat60°C;StoredinDNAstablePlusat85°C;Storedunprotected(TE)at60°C;andStoredunprotected(TE)at85°C(noDNArecovered).Thecontrolwasaliquidwhereasallothersampleswererecoveredfromadriedstate.
Figure6:qPCRtracesofDNArecoveryexperiments,describedaboveinFigure5.Representativetracesfromtriplicatesamplesareshown.
Figure7:DNArecoveredafterstorageat+85°Cfor3monthsindriedformat.SamplesprotectedwithDNAstablePlus(DSP)arerecoveredsimilarto-20°Ccontrols.However,non-protected(NP)samplesandcompetition“G”shownoDNArecovery.
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Specifications
- ProductFormats:2mland10mlLiquidReagents
- Applicationvolume:1:4(vol:vol)ofstabilizer:liquidDNA
- Applicationamount:0.001to250µg
- Dryingtime:10min(SpeedVac)toovernight(Laminarhood)
- Recoveryvolume:10to200µl
- Recovery:~99.9%
- DNAstorageinliquidatRT:1year(acceleratedstudies,6monthsforplasmidDNA)
- DNAstorageindriedformatatRT:Atleast40years(acceleratedstudies)
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