RNAandDNAFromOneSample
- BestyieldandqualityforbothRNA&DNA
- SuperiortranscriptomestABIlity&fidelity
- Shipsampleswithoutcoldchain
- Proveninhigh-throughputworkflows
Product
PreserveRNAandDNAInstantly
TheRNAgardBloodSystemisdesignedforthecollection,preservationandpurificationofRNAandDNAfromwholebloodsamples.TheRNAgardBloodpreservativereagentinthetubesrapidlypermeatesandlysescellularstructuresandmembranestostabilizeandprotectRNAandDNAfromdegradation.Preservationiseffectivefor14daysatroomtemperatureor1monthat4°C.
ProductComparison
| Properties* | RNAgard | CompetitorP |
|---|---|---|
| RNAYield | ||
| RNAQuality(RIN) | ||
| TranscriptomeStability** | ||
| RNA&DNAfromSameTube(High-Throughput) | ||
| *Samplestestedafter2weeksat25°C,7Weeksat4°C **byIlluminaHumanHT-12v4arraywith>47Ktranscripts | ||
SaveSave
SaveSave
SaveSave
SaveSave
SaveSave
Data
ProtectRNAinstantlyatambienttemperatures
Figure1.GeneexpressionanalysisofbloodRNApreservationafter3and14daysinRNAgardBlood(RGB).Humanbloodfrom2healthydonorswascollectedinRNAgardtubesorcompetitor’sRNApreservationtubes.RNAwasisolatedoncollectiondayandafter3or14daysofroomtemperaturestorage.Top:Representativeanalysis,forDonor2,ofgeneexpressionprofileofmorethan47000transcripts,usingtheIluminaHumanHT12BeadArray,forRNAextractedfrombloodcollectedinRNAgardBloodorcompetitor’sTubesafter3days(A,C)or14days(B,D)ofroomtemperaturestorage,relativetoRNAextractedfromfreshlycollectedblood,usingRNAisolationmethodsrecommendedforeachproduct.Bottom:Tablesummarizingthemicroarrayresults.Displayedarethenumberofgeneswith>2-foldchangeinexpressionlevelsrelativetoexpressionlevelsonthedayofbloodsamplecollection.
RNAyieldfromBioMaxiBloodRNAPurificationKit
Figure2.RNAyieldanalyzedbyUVspectroscopy.RNAamountsareforwholetube(2.5mLblood)isolatingusingBioMaxiBloodRNAPurificationkitafter14daysofstorageatambienttemperature.
14-dayRNAprotectionatambienttemperatures
Figure3.MultipledonorcomparisonofRNAgardBloodTubes(RGB)versusCompetitorP(P)after14daysofambienttemperaturestorage.200ngofRNAisvisualizedona1.2%agarosegel.BioMaxiBloodRNAPurificationkitwasusedforisolatingRNAfromRNAgardBloodTubes.
RNAgardBloodSystem:ReproducIBLeandRepeatableRNAPurification:RNAPurity(A260/A280)for3Operators(A,B,C)
Figure4.RNApurityobtainedusingtheRNAgardBloodSystemishighlyreproducible.Bloodwascollectedfrom8healthydonors.After3,7or15daysofsamplestorage,RNAwaspurifiedfromduplicatesamples.RNApurityofeachindividualsamplewasdeterminedbyUVspectrophotometry(A260/A280).OnDay3,duplicatesamplesforeachdonorwerepurifiedby3differentoperatorsshowingreproducibilityofoursystem.
StabilityofspecificRNAtranscriptsisolatedwithRNAgardBloodSystem
Figure5.RNAgardBloodTubemaintainsunalteredgeneexpressionfor14daysat18-25°C.BloodfromtwohealthydonorswascollectedinRNAgardtubesandpurifiedwithBioMaxiRNAaftertheindicatedstoragetime.GeneexpressionofC-Fos(A)andIL1-β(B),normalizedto18SrRNA,wasdeterminedbyqPCRattheindicatedstoragetime,relativetoday0samples.
RNAStabilityafter7weeksat4°C
Figure6.RNAyieldofwholebloodfrom5differentdonorswastestedusingtheRNAgardBloodSystemfollowingstoragefor7weeksat4°Cversuscontrol(Day=0).DatashowsthatthequalityandyieldofRNAfollowing7weeksofstorageinRNAgardBloodTubesat4°CwereequivalenttothatofRNApurifiedfromwholebloodonthedayofblooddraw.
Impactoffreeze/thawconditionsontheintegrityofRNAgardBloodTubes
Table1.RNAgardBloodTubesremainintactafterfreezerstoragefor7days.BloodwascollectedfromtwohealthydonorsintoRNAgardBloodTubes.Thetubeswerethenkeptatroomtemperatureforatleast2hoursbeforefreezingdown.Fourdifferentfreezingmethodsweretested,andthetubeswerestoredfrozenasindicatedfor7days.Thetubeswerethawedusingthreedifferentmethods.Notubedamages(cracks,splits,etc.)wereobserved.
Stablegeneexpression>47,000transcripts
TestedonIlluminaHumanHT-12v4Array
TestedonIlluminaHumanHT-12v4Array
GeneTranscript:BCR-ABL
FunctionalTest:RT-qPCR
Functionaltestingwithageneexpression-basedcancerdiagnostictest.
RNAyield(70%higherwithRNAgard)
RNAQuality
RNAIntegrity
SaveSave
SaveADIus:2px;border-top-right-radius:2px;border-bottom-right-radius:2px;border-bottom-left-radius:2px;text-indent:20px;width:auto;padding:0px4px0px0px;text-align:center;font-style:normal;font-variant-caps:normal;font-weight:bold;font-stretch:normal;font-size:11px;line-height:20px;font-family:"HelveticaNeue",Helvetica,sans-serif;color:#ffffff;background-image:url(data:image/svg+xml;base64,phn2zyb4bwxucz0iahr0cdovl3d3dy53my5vcmcvmjawmc9zdmciighlawdodd0imzbwecigd2lkdgg9ijmwchgiihzpzxdcb3g9ii0xic0xidmxidmxij48zz48cgf0acbkpsjnmjkundq5lde0ljy2miBDmjkundq5ldiyljcymiaymi44njgsmjkumju2ide0ljc1ldi5lji1nibdni42mzismjkumju2idaumduxldiyljcymiawlja1mswxnc42njigqzaumduxldyunjaxidyunjmyldaumdy3ide0ljc1ldaumdy3iemymi44njgsmc4wnjcgmjkundq5ldyunjaxidi5ljq0oswxnc42njiiigzpbgw9iinmzmyiihn0cm9rzt0ii2zmziigc3ryb2tllxdpzhropsixij48l3bhdgg+phbhdgggzd0itte0ljczmywxljy4nibdny41mtysms42odygms42njusny40otugms42njusmtqunjyyiemxljy2nswymc4xntkgns4xmdksmjquodu0idkuotcsmjyunzq0iem5ljg1niwyns43mtggos43ntmsmjqumtqzidewljaxniwymy4wmjigqzewlji1mywymi4wmsaxms41ndgsmtyuntcyidexlju0ocwxni41nzigqzexlju0ocwxni41nzigmteumtu3lde1ljc5nsaxms4xntcsmtqunjq2iemxms4xntcsmtiuodqyideyljixmswxms40otugmtmuntiyldexljq5nsbdmtqunjm3ldexljq5nsaxns4xnzusmtiumzi2ide1lje3nswxmy4zmjmgqze1lje3nswxnc40mzygmtqundyylde2ljegmtqumdkzlde3ljy0mybdmtmunzg1lde4ljkznsaxnc43ndusmtkuotg4ide2ljayocwxos45odggqze4ljm1mswxos45odggmjaumtm2lde3lju1niaymc4xmzysmtqumdq2iemymc4xmzysmtauotm5ide3ljg4ocw4ljc2nyaxnc42nzgsoc43njcgqzewljk1osw4ljc2nya4ljc3nywxms41mzygoc43nzcsmtqumzk4iem4ljc3nywxns41mtmgos4ymswxni43mdkgos43ndksmtcumzu5iem5ljg1niwxny40odggos44nzismtcunia5ljg0lde3ljczmsbdos43ndesmtgumtqxidkuntismtkumdizidkundc3lde5ljiwmybdos40miwxos40nca5lji4ocwxos40otegos4wncwxos4znzygqzcunda4lde4ljyymia2ljm4nywxni4yntigni4zodcsmtqumzq5iem2ljm4nywxmc4yntygos4zodmsni40otcgmtuumdiyldyundk3iemxos41ntusni40otcgmjmumdc4ldkunza1
